Efficacy of different combinations of microbial biocontrol agents against sheath blight of rice caused by Rhizoctonia solani

Abstract Background Sheath blight of rice caused by Rhizoctonia solani is one the most important diseases worldwide, causing considerable yield losses. The estimation losses due to sheath in India has been reported be up 54.30%. As a consequence this fact, eco-friendly approaches were explored investigation. Results pathogen R. was isolated from infected plant, which identified and characterized on basis morphology through molecular Sequencing. sequences ITS submitted NCBI GenBank accession number allotted SUB11543577. In dual culture best two potential isolates biocontrol agents selected Pseudomonas fluorescens (Pf27) Trichoderma harzianum (Th47). Their different combinations with Herbal Kunapajala (HKJ) tested glasshouse, experimental field farmers’ fields against solani. maximum plant vigor index found treatment combination P. (27) + T. harzanium (47) Kunapajala, seed treatment, soil drenching three foliar applications. Minimum disease severity incidence at 30 days after sowing (DAS) 60DAS, Phenylalanine Ammonia Lyase Activity (PAL), Polyphenol Oxidase (PPO) Peroxidase (PO) also recorded same treatment. experiment population 60 transplanting cfu/g for spp . (32.33 × 10 4 ) (36.33 rhizosphere rhizoplane. Maximum (24.67 spp. (24.0 observed Th Pf HKJ [seed spray]. At farmers minimum severity, DAT spray] par chemical Carbendazim, but obtained PF growth promotion activity. Conclusions Among various sprays (47), very effective reducing incidence, increasing activity all conditions. Therefore, recommendation investigation could exploited under bio-intensive management program sustainable cultivation rice.